Molecular hybridization with DNA-probes as a laboratory diagnostic test for influenza viruses.
Identifieur interne : 002E51 ( Main/Exploration ); précédent : 002E50; suivant : 002E52Molecular hybridization with DNA-probes as a laboratory diagnostic test for influenza viruses.
Auteurs : A Z Pljusnin ; S A Rozhkova ; O V Nolandt ; E A Bryantseva ; O K Kuznetsov ; F S NoskovSource :
- Virologie [ 0253-181X ]
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- diagnostic : Grippe humaine.
- microbiologie : Grippe humaine.
- ADN, Adolescent, Adulte, Enfant, Humains, Hybridation d'acides nucléiques, Orthomyxoviridae.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- chemical : DNA.
- classification : Orthomyxoviridae.
- diagnosis : Influenza, Human.
- microbiology : Influenza, Human.
- Adolescent, Adult, Child, Humans, Nucleic Acid Hybridization.
Abstract
The possibilities of using DNA-copies of different influenza A virus genes cloned with recombinant bacterial plasmids for the detection of virus-specific RNA by molecular dot-hybridization were analyzed. High specificity of RNA identification has been demonstrated and it has been shown expedient to use DNA-probes with high-conservative virus genes (polymerase, nucleoprotein, or matrix) for the detection of influenza A virus subtypes (H1N1, H2N2, H3N2) and probes with corresponding hemagglutinin genes for the differentiation of the subtypes H3N2 and H1N1. The results of nasopharyngeal specimens testing proved the effectiveness of molecular dot-hybridization in epidemiological studies of influenza outbreaks, especially of mixed etiology.
PubMed: 3617494
Affiliations:
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Le document en format XML
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Noskov</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>1987 Apr-Jun</MedlineDate></PubDate></date><idno type="RBID">pubmed:3617494</idno><idno type="pmid">3617494</idno><idno type="wicri:Area/PubMed/Corpus">000487</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000487</idno><idno type="wicri:Area/PubMed/Curation">000487</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000487</idno><idno type="wicri:Area/PubMed/Checkpoint">000636</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000636</idno><idno type="wicri:Area/Ncbi/Merge">001079</idno><idno type="wicri:Area/Ncbi/Curation">001079</idno><idno type="wicri:Area/Ncbi/Checkpoint">001079</idno><idno type="wicri:doubleKey">0253-181X::Pljusnin A:molecular:hybridization:with</idno><idno type="wicri:Area/Main/Merge">003026</idno><idno 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sort="Noskov, F S" uniqKey="Noskov F" first="F S" last="Noskov">F S Noskov</name></author></analytic><series><title level="j">Virologie</title><idno type="ISSN">0253-181X</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Child</term><term>DNA</term><term>Humans</term><term>Influenza, Human (diagnosis)</term><term>Influenza, Human (microbiology)</term><term>Nucleic Acid Hybridization</term><term>Orthomyxoviridae (classification)</term><term>RNA, Viral (analysis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN</term><term>ARN viral (analyse)</term><term>Adolescent</term><term>Adulte</term><term>Enfant</term><term>Grippe humaine (diagnostic)</term><term>Grippe humaine (microbiologie)</term><term>Humains</term><term>Hybridation d'acides nucléiques</term><term>Orthomyxoviridae ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Child</term><term>Humans</term><term>Nucleic Acid Hybridization</term></keywords><keywords scheme="MESH" 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High specificity of RNA identification has been demonstrated and it has been shown expedient to use DNA-probes with high-conservative virus genes (polymerase, nucleoprotein, or matrix) for the detection of influenza A virus subtypes (H1N1, H2N2, H3N2) and probes with corresponding hemagglutinin genes for the differentiation of the subtypes H3N2 and H1N1. The results of nasopharyngeal specimens testing proved the effectiveness of molecular dot-hybridization in epidemiological studies of influenza outbreaks, especially of mixed etiology.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Bryantseva, E A" sort="Bryantseva, E A" uniqKey="Bryantseva E" first="E A" last="Bryantseva">E A Bryantseva</name><name sortKey="Kuznetsov, O K" sort="Kuznetsov, O K" uniqKey="Kuznetsov O" first="O K" last="Kuznetsov">O K Kuznetsov</name><name sortKey="Nolandt, O V" sort="Nolandt, O V" uniqKey="Nolandt O" first="O V" last="Nolandt">O V Nolandt</name><name sortKey="Noskov, F S" sort="Noskov, F S" uniqKey="Noskov F" first="F S" last="Noskov">F S Noskov</name><name sortKey="Pljusnin, A Z" sort="Pljusnin, A Z" uniqKey="Pljusnin A" first="A Z" last="Pljusnin">A Z Pljusnin</name><name sortKey="Rozhkova, S A" sort="Rozhkova, S A" uniqKey="Rozhkova S" first="S A" last="Rozhkova">S A Rozhkova</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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